Ali ghavampour; Mohammad Ali Salari Aliabadi; Hossein Zolgharneen; bita archangi
Abstract
Resource genetics idendtification is one of the most efficient strategies for fisheries management regarding commercially important marine resources.One of the valuable species of shrimp in the Persian Gulf is the banana shrimp (Fenneropenaeus merguiensis), which accounts for about 60% of total annual ...
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Resource genetics idendtification is one of the most efficient strategies for fisheries management regarding commercially important marine resources.One of the valuable species of shrimp in the Persian Gulf is the banana shrimp (Fenneropenaeus merguiensis), which accounts for about 60% of total annual shrimp catch in Hormozgan province.Identifying the genetic structure of the country's marine reserves is an effective way of efficient fisheries management. In this study, the amount of genetic diversity in the populations of this species was studied using microsatellite markers by 10 primers in four populations at Jask, Kolahi, Bandar Abbas power plant and Tula catch area and their genetic characteristics were compared with a 1 gram shrimp batch as a sample from stock enhancement project which was being stocked near kolahi area. Results of Genetic Distance Matrix, Heterozygosity, Assignment Test and Hardy-Weinberg Equilibrium at the Locations Indicated Average Population Distance (over 0.05 ) and Possible Effects of nursery ground and Regional Hydrodynamic Flows.
علوم زیستی دریا
Zahra Yarahmadi; Bita Archangi; Ahmad Savari; Mohammad Bagher Nabavi
Abstract
The objective of this study was to morphological description and phylogenetic study of Ornithocercus magnificus in Bandar Abbas waters. The genus Ornithocercus is a heterotrophic dinoflagellate of the family Dinophysaceae. The samples were collected using a phytoplankton net (mesh size: 20 µm) ...
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The objective of this study was to morphological description and phylogenetic study of Ornithocercus magnificus in Bandar Abbas waters. The genus Ornithocercus is a heterotrophic dinoflagellate of the family Dinophysaceae. The samples were collected using a phytoplankton net (mesh size: 20 µm) equipped with a flowmeter in surface waters. The plankton samples were scanned and identified with an inverted microscope. Then, the SSU rDNA gene fragment from individual cells have been amplified using the polymerase chain reaction and sequenced for the Iranian strain of this species. Phylogenetic analyzes were performed by Maximum Likelihood (ML) and Maximum parsimony (MP) methods. In this study, two new sequences of SSU rDNA from O. magnificus were reported for the first time and deposited in the GenBank. Morphological studies showed that the isolated cells from Iranian water were O. magnificus. Generally, the results showed; the morphological results of O. magnificus were matched with the molecular results of this study. Phylogenetic analysis grouped isolated Iranian sequences with O. magnificus and formed a monophyletic clad with other members of this genus as sister groups. The genus Histioneis is the closest relative to Ornithocercus.
علوم زیستی دریا
Maryam GhasemiTirtash; Ahamd Savari; bita archangi; Nasrin Sakhaei; Neda Mehdipour
Abstract
Monitoring of benthic communities is important factor in ecosystem health studies. The Caspian Sea is considered as an independent zoogeographical region due to its diversity, specificity and endemism of its fauna. For studying frequency and distribution patterns of macrobenthic community, 5 transects ...
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Monitoring of benthic communities is important factor in ecosystem health studies. The Caspian Sea is considered as an independent zoogeographical region due to its diversity, specificity and endemism of its fauna. For studying frequency and distribution patterns of macrobenthic community, 5 transects along the Southern Coasts of Caspian Sea from Astara to Chaboksar were sampled. In each transect 3 stations with the depth of 1,5 and 10 meters were selected in perpendicular line to the beach .Physical parameters such as Temperature, Salinity, Dissolved Oxygen and pH were measured in addition to sediment grain size. Sampling has done twice a year (winter 2014 and summer 2015).Totally 38 macro benthos species were identified. Gastropods of Hydrobiidae family and polychaet Streblospio gynobranchiata were the dominant group with the contribution of 80% of total macrobenthos population. Average number of total benthos was 2250 individual/m2 in winter and 2630 individual/m2 in summer. Non-parametric statistical tests showed significant difference between number and frequency of species in different depths (p
علوم زیستی دریا
maryam moazami; Mohammad Ali Salari-Aliabadi; bita archangi; Hossein Zolgharnein; Ahmad ghasemi
Abstract
In this study, genetic diversity of Pinctada persica population was evaluated using PCR-RFLP method. To determine and compare genetic diversity of P. persica populations in khark, shidvar, hendurabi and larak islands, 51 samples were collected from 1-6 m depths. DNA of samples was extracted by CTAB method. ...
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In this study, genetic diversity of Pinctada persica population was evaluated using PCR-RFLP method. To determine and compare genetic diversity of P. persica populations in khark, shidvar, hendurabi and larak islands, 51 samples were collected from 1-6 m depths. DNA of samples was extracted by CTAB method. A pair of primers of Pinctada persica was used for polymerase chain reaction. Enzymatic digestion of PCR yield was performed using, AvaΙΙ, DdeΙ, HindIII, SfaNΙ and TaqΙ restriction enzymes. DNAs were collected and stained with silver staining after subjected to poly acrylamide gel electrophoresis The results revealed that electrophoresis and enzymatic digestion fragments of the 51 samples collected from four stations in the northern coast of Persian Gulf with all 5 enzymes were the same The results of this study confirm that Pinctada persica Based on the studied gene is completely homogenous in these stations and show no genetic diversity.
علوم زیستی دریا
N Nozarpour; M.B Nabavi; Ronagh M.T Ronagh; B Archangi; N Sakhai
Abstract
In this study, the molecular characteristics of Chiromantes boulengeri in 2014 from intertidal waters of Arvand River in the vicinity of Minoo island were investigated. So, in October 2014 specimens from intertidal of two area of Minoo island were collected and preserved in 70% ethanol, carried out to ...
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In this study, the molecular characteristics of Chiromantes boulengeri in 2014 from intertidal waters of Arvand River in the vicinity of Minoo island were investigated. So, in October 2014 specimens from intertidal of two area of Minoo island were collected and preserved in 70% ethanol, carried out to the khoramshahr University of Marine Science and Technology laboratory and examined. To fulfill the purpose, To extract the DNA, phenol- chloroform method were used and mitochondrial gene 16S rRNA was sequenced and compare with other species from GenBank NCBI. The morphological and phylogeny sequencing results showed that all specimens belong to Chiromantes boulengeri in the study area. By polymorphism analysis, there are mutation within C. boulengeri from two area of Arvand River and it seems that this species for its survival has adapted with different ecological conditions in the study area.
علوم زیستی دریا
Esmat Salimi; hossein zolgharnein; Bita Archangi; Mohammad Taghi Ronagh; Seyyed Ahmad Ghasemi
Abstract
In this study, two populations of Aphanius ginaonis (Holly, 1929) and Aphanius dispar (Ruppell, 1828) were examined to determine genetic diversity using PCR-RFLP technique. A total of 60 individual specimens were collected from Hormozgan and Bushehr internal waters, comprising two sampling sites; from ...
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In this study, two populations of Aphanius ginaonis (Holly, 1929) and Aphanius dispar (Ruppell, 1828) were examined to determine genetic diversity using PCR-RFLP technique. A total of 60 individual specimens were collected from Hormozgan and Bushehr internal waters, comprising two sampling sites; from Geno hot spring (30 individuals of A. ginaonis) and Mir Ahmad hot spring (30 individuals of A. dispar). The D-loop region of mitochondrial DNA (550 bp) was amplified using PCR followed by RFLP analysis based on 5 restriction endonucleas enzymes (AluI, DpnI, Eco47I, HindIII HinfI). In order to undertake data analysis, Arlequin 3.11 was applied. Results at population levels indicated that Geno individuals have more haplotype diversity than Mir Ahmad individuals (9 and 3 haplotypes respectively). However, further investigation using genetic techniques is required to clarify the molecular history and evolution of Aphanius species in this area. Results obtained from this research would be applicable to understand conservation genetics and management of this important fish species in Iran.
علوم زیستی دریا
, A iTaherpour; bita archangi; S Ghaemmaghami; H Zolgharnein; K Ghanemi
Abstract
Brown algae of Padina sp (Adanson, 1763) are found globally across tropical coastal areas, inter-tidal and sub-tidal regions. The aim of this research was to identify morphological and molecular characteristics of padina species distributed across Lengeh Port in Persian Gulf using morphological examinations ...
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Brown algae of Padina sp (Adanson, 1763) are found globally across tropical coastal areas, inter-tidal and sub-tidal regions. The aim of this research was to identify morphological and molecular characteristics of padina species distributed across Lengeh Port in Persian Gulf using morphological examinations and rbcL chloroplast gene sequencing. For this purpose, morphological features were undertaken using valid identification keys. For molecular analysis, total genomic DNA was extracted by CTAB method with slight modifications. Amplification of fragments was carried out using rbcL primers. Analysis of genome sequences was undertaken using genetic softwares of Chromas, BioEdit and MEGA6 and phylogenetic trees were constructed by two models of Neighbor Joining (NJ) and Maximum Likelihood (ML). Results indicated that two identical species of padina (P. boergesenii and P. australis) in this research shared common cluster with 99% probability and showed high genetic similarities among related compared padina species submitted in NCBI Genbank.
علوم زیستی دریا
Sharareh Savari; a s; b a; a s; r a
Abstract
Epinephelus coioides fish is cultivated in Emam Khomeini Fisheries Research Center under Southern Aquaculture division. This fish is used in histology, histopathalogy, toxicology, physiology, cellular and molecular biology, studies. The Zangi creek water is contaminated due to petrochemical, industrial, ...
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Epinephelus coioides fish is cultivated in Emam Khomeini Fisheries Research Center under Southern Aquaculture division. This fish is used in histology, histopathalogy, toxicology, physiology, cellular and molecular biology, studies. The Zangi creek water is contaminated due to petrochemical, industrial, agricultural and commercial communication pollutants. The water that the fish are cultivated with is obtained from Zangi creek and the fish which are exposed to this water are contaminated. In the histopathological experiments performed by Haemotoxylin- Eosin staining method in the brain, disorders such as karyolysis and necrosis of the nucleus, vaculation and oedema as well as hyperanemia are apparent. These defects are more obvious in the medulla oblongata, diencephalon and cerebellum of the brain. These disorders could be due to contamination of Zangi creek water such as heavy metals, organic derivatives of heavy metals, aromatics, organophosphates and etc.
علوم زیستی دریا
f kh; Hossein Zolgharnien; Bita Archangi; Ebrahim Rajabzadeh; Ahmad Qasemi
Abstract
According to the importance of growth hormone gene in aquaculture, in this study, cloning of gowth hormone gene of Orange-spotted grouper (Epinephelus coioides) was examined in pTZ57R/T vector.For this purpose, growth hormone cDNA was preparated and amplified using specific designed primers. PCR product ...
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According to the importance of growth hormone gene in aquaculture, in this study, cloning of gowth hormone gene of Orange-spotted grouper (Epinephelus coioides) was examined in pTZ57R/T vector.For this purpose, growth hormone cDNA was preparated and amplified using specific designed primers. PCR product was purified by QIAquick Gel Extraction Kit, Growth hormone gene was inserted in pTZ57R/T vector. Ligation product was transformed into competent E.coli (DH5α) cells. The white clones selected on the LB media and were extracted plasmid as recombinant bacteria .verified clones obtained in this study were performed by direct PCR and sequencing methods Orange-spotted grouper growth hormone cDNA contains an open reading frame consisting of 615 nucleotides and 204 amino acid protein. The calculated molecular weight and isoelectric point of growth hormone protein were respectively 23.014 and 6.9. Results of this study suggested that growth hormone gene of Orange-spotted grouper was successfully cloned in the pTZ57R/T vector, and can be used for growth hormone gene expression in expression vectors and growth hormone protein production. Comparison of gene sequences obtained with E.coioides GH gene sequence in gene bank, indicate sequence similarity between them.
zeynab ahmadi; hossein zolgharein; bita archangi; ebrahim rajabzade
Abstract
16S rRNA is one of the components of the ribosome and widely used in phylogenetic studies of closely related species and especially related species Crassostrea. Nucleotide sequence polymorphism associated with 16S rRNA gene of mitochondrial genome of Persian Gulf Oyster (Crassostrea sp.), examined in ...
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16S rRNA is one of the components of the ribosome and widely used in phylogenetic studies of closely related species and especially related species Crassostrea. Nucleotide sequence polymorphism associated with 16S rRNA gene of mitochondrial genome of Persian Gulf Oyster (Crassostrea sp.), examined in 30 samples collected from Badar-e Emam Khomeini oysters. Through a primer PCR chain reaction was performed for all samples and product of PCR were Automated sequencing. Then the data obtained were analyzed by the programs and software. For the gene studied only there was 2 mutation and Nucleotide diversity (Pi) 0.00061 calculated. Among the 30 samples, 3 haplotypes were detected and average haplotype diversity (Hd) 0.195 were calculated. The haplotypes obtained in this study were first registered in the gene bank. Phylogeny trees drived from 16S rRNA gene sequencing showed no clear separation of the samples in the Bandar-e Imam Khomeini region. The results of this study showed that there is low sequence differentiation in the study population in Bandar-e Imam Khomeini.
bita archangi
Abstract
In this study, effects of BPA on erythrocytic nuclear abnormalities (ENA) stimulus (MN Test) and liver DNA integrity (DNA Unwinding Assay), in male yellowfin seabream (Acanthopagrus latus) were investigated. For this reason, fish received intraperitoneal injections during a period of 2 weeks with 10, ...
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In this study, effects of BPA on erythrocytic nuclear abnormalities (ENA) stimulus (MN Test) and liver DNA integrity (DNA Unwinding Assay), in male yellowfin seabream (Acanthopagrus latus) were investigated. For this reason, fish received intraperitoneal injections during a period of 2 weeks with 10, 50, 100 and 150 µg g-1 week-1 of BPA dissolved in coconut oil. Solvent controls received the coconut oil whereas controls were not injected. The fish were sampled on day 0, 7 and 14. In order to evaluate cytotoxicity of BPA presented in blood, the erythrocytic nuclear abnormalities (ENA) frequency in yellowfin seabrem was determined. Our results demonstrated a significant increase in fish micronuclei frequency after the treatment with bisphenol A in comparison to the control groups and in dose dependent manner. In addition, the rate of liver DNA integrity was tested using the DNA alkaline unwinding assay. Results showed a decrease in the rate of liver DNA integrity in treated fishes after 7 and 14 days of BPA exposure in comparison to the control group. In conclusion, results of the current project indicated that BPA has high genotoxic and/or cytogenotoxic potential. It could be concluded that Micronucleus test and DNA strand breaks can be used as sensitive cellular and molecular indicators of exposure to genotoxic BPA.
Alireaza Safahieh; Sahel Pakzadtoochaei; Mohammad Taghi Ronagh; Bita Archangi; Mohammad Ali Hamzeh
Volume 12, Issue 3 , September 2014, , Pages 15-25
Abstract
Heavy metals in marine ecosystems are from both natural and anthropogenic sources. The monsoon is a natural phenomenon that can effect the heavy metals concentration. The monsoon can affect heavy metal concentration in coastal areas through rainfall and leaching and also disrupting of water layer. The ...
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Heavy metals in marine ecosystems are from both natural and anthropogenic sources. The monsoon is a natural phenomenon that can effect the heavy metals concentration. The monsoon can affect heavy metal concentration in coastal areas through rainfall and leaching and also disrupting of water layer. The effect of monsoon on changes of concentrations heavy metals such as Cd, Cu, Ni, Pb and Zn in the sediment of Chabahar coast were studied in 2010. Samples were collected from the sediment of Gwatr, Beris, Ramin, Chabahar, Tis and Pozm stations in premonsoon, monsoon and post monsoon seasons (May, August and November respectively) during low tide. After preparation of samples, heavy metal concentration was determined by using the atomic absorption spectrophotometer. The concentration of Cd, Cu, Ni, Pb and Zn were 0.36, 4.97, 17.14, 8.88 and 24.93 µg/g dw respectively. The Cd concentration in the sediment was influenced by regional sources. The increase of Cu and Zn concentration from monsoon until post monsoon could be caused by rainfall and disturbance in the coastal sediments. The increase of Pb concentration in monsoon and post monsoon could be due to rapid precipitations of Pb, this increase could be affected due to coastal runoff in monsoon and the resumption of the vessel’s activity in post monsoon.
m r; h z; v y; m s; b a
Volume 12, Issue 4 , September 2014, , Pages 40-50